The B5366 heads south from Leicester city centre. It was originally unclassified but gained its number in the late 1920s.
The road starts at a junction with the A426 at Freemen's Common to the south of the city centre, not far from the Walker Stadium. From here it heads south in a shallow cutting passing beneath a freight-only railway line and then through an area of extensive council housing to meet the A563 and the start of the B5418 at Pork Pie Island, Southfields.
Continuing south, it crosses first the Leicester City boundary and then the Leicester to Birmingham railway line near South Wigston station before terminating on the B582 in South Wigston itself.